Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29853 | 5' | -54.9 | NC_006273.1 | + | 125386 | 0.66 | 0.966743 |
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Target: 5'- uUGCuGCAGCgGGAUGAUUUCG-CCu -3' miRNA: 3'- gACGcUGUCGaCCUACUGGAGUaGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 101367 | 0.66 | 0.966743 |
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Target: 5'- -gGCGGCGGCc--GUGcgcaucgccuGCCUCAUCCGa -3' miRNA: 3'- gaCGCUGUCGaccUAC----------UGGAGUAGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 44648 | 0.66 | 0.969441 |
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Target: 5'- -gGCGGCAGCUGG-UGgcguucuGCCUggagaaggugugCGUCCGc -3' miRNA: 3'- gaCGCUGUCGACCuAC-------UGGA------------GUAGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 21376 | 0.66 | 0.972521 |
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Target: 5'- -gGCGACcGuCUGGAggUGGCCUgCAUCUu -3' miRNA: 3'- gaCGCUGuC-GACCU--ACUGGA-GUAGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 193086 | 0.66 | 0.972521 |
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Target: 5'- gCUG-GACGGCUGGAgcugGAUCgccaGUCCc -3' miRNA: 3'- -GACgCUGUCGACCUa---CUGGag--UAGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 58474 | 0.66 | 0.975121 |
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Target: 5'- gUGUGAUgaAGCUGGAgagcUGGgCUCAcgucuUCCGg -3' miRNA: 3'- gACGCUG--UCGACCU----ACUgGAGU-----AGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 148678 | 0.66 | 0.975121 |
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Target: 5'- uCUGCGcgcaGCuGCUGGAgcuCCUCGUCg- -3' miRNA: 3'- -GACGC----UGuCGACCUacuGGAGUAGgc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 68424 | 0.66 | 0.975121 |
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Target: 5'- -aGCGGCGGaCUGGAcgUGACCacccCCGa -3' miRNA: 3'- gaCGCUGUC-GACCU--ACUGGaguaGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 206002 | 0.66 | 0.977537 |
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Target: 5'- cCUGCGACGuGC-GcGAcGACCgcuaCAUCCGg -3' miRNA: 3'- -GACGCUGU-CGaC-CUaCUGGa---GUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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