Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29853 | 5' | -54.9 | NC_006273.1 | + | 162821 | 0.67 | 0.956546 |
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Target: 5'- -cGCcGCAGCUGcGG-GGCCUCAUCg- -3' miRNA: 3'- gaCGcUGUCGAC-CUaCUGGAGUAGgc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 163426 | 0.68 | 0.939907 |
|
Target: 5'- -cGCGugGGCUGGAaagccgugcugGGCCUCAaCgCGg -3' miRNA: 3'- gaCGCugUCGACCUa----------CUGGAGUaG-GC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 164628 | 0.67 | 0.944403 |
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Target: 5'- -aGCGGgaAGgUGGGgaaGGCCUCGUCCa -3' miRNA: 3'- gaCGCUg-UCgACCUa--CUGGAGUAGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 182148 | 0.67 | 0.963553 |
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Target: 5'- -cGCGugAGUUGGGUGguucACCgUCAggugcUCCGa -3' miRNA: 3'- gaCGCugUCGACCUAC----UGG-AGU-----AGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 185853 | 0.67 | 0.956546 |
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Target: 5'- -gGaCGACGGUa-GAUGACCUCGUCUu -3' miRNA: 3'- gaC-GCUGUCGacCUACUGGAGUAGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 192989 | 0.69 | 0.908123 |
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Target: 5'- -aGCGAC-GCgacuuuggGGAUGGCCUCGUgCu -3' miRNA: 3'- gaCGCUGuCGa-------CCUACUGGAGUAgGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 193086 | 0.66 | 0.972521 |
|
Target: 5'- gCUG-GACGGCUGGAgcugGAUCgccaGUCCc -3' miRNA: 3'- -GACgCUGUCGACCUa---CUGGag--UAGGc -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 206002 | 0.66 | 0.977537 |
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Target: 5'- cCUGCGACGuGC-GcGAcGACCgcuaCAUCCGg -3' miRNA: 3'- -GACGCUGU-CGaC-CUaCUGGa---GUAGGC- -5' |
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| 29853 | 5' | -54.9 | NC_006273.1 | + | 227852 | 0.7 | 0.860865 |
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Target: 5'- gUGCGAgAGUacacgaUGGGUGGCuCUCGUCuCGg -3' miRNA: 3'- gACGCUgUCG------ACCUACUG-GAGUAG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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