Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2989 | 5' | -57.4 | NC_001493.1 | + | 23084 | 0.66 | 0.837809 |
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Target: 5'- cGUgaccaGGUUCCACGGgGUacgacccggcuucgaGACCGAGGc -3' miRNA: 3'- cCGag---UCAAGGUGCCgCG---------------CUGGCUCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 18061 | 0.68 | 0.754443 |
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Target: 5'- gGGCggUCGGgggCCugGGUGCcGCCGuGAa -3' miRNA: 3'- -CCG--AGUCaa-GGugCCGCGcUGGCuCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 17763 | 0.69 | 0.685913 |
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Target: 5'- gGGgUCGGggCCGCaacgcgGGCGUGcCCGGGAu -3' miRNA: 3'- -CCgAGUCaaGGUG------CCGCGCuGGCUCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 17652 | 0.67 | 0.800404 |
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Target: 5'- aGGcCUCcgugccgauggGGUUCCAgaagaaGGUGCGAUCGGGGu -3' miRNA: 3'- -CC-GAG-----------UCAAGGUg-----CCGCGCUGGCUCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 10866 | 0.7 | 0.615224 |
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Target: 5'- cGGCgaUCGGUgaCCGCGGCcgguucGCGAuCCGGGAc -3' miRNA: 3'- -CCG--AGUCAa-GGUGCCG------CGCU-GGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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