miRNA: 3'- -CC-GAGUCaaGGU---GCC-GCGCUGGCUCU- -5'
miRNA: 3'- cCgAGUCAAGGUGCCgc-GCUGGCuCU- -5'
miRNA: 3'- -CCGAGUCa--AGG--UG-CCGC-GCUGGCUCU- -5'
miRNA: 3'- -CCGAGUCa--AGGUGCCGCGCUg-GCUcu -5'
miRNA: 3'- -CCGAGUCAAGGUGCCGCGCUGGCUCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2989 | 5' | -57.4 | NC_001493.1 | + | 32576 | 0.71 | 0.574929 |
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Target: 5'- gGGuCUCGGg-CCGgacCGGuCGCGACCGGGAc -3' miRNA: 3'- -CC-GAGUCaaGGU---GCC-GCGCUGGCUCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 24720 | 0.71 | 0.564938 |
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Target: 5'- cGgUCAGUUCCACGGgaaCGGCCGuGAc -3' miRNA: 3'- cCgAGUCAAGGUGCCgc-GCUGGCuCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 62227 | 0.75 | 0.357146 |
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Target: 5'- cGGCUCAGcuaUCCcaACgGGCGaCGACCGGGGc -3' miRNA: 3'- -CCGAGUCa--AGG--UG-CCGC-GCUGGCUCU- -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 25860 | 0.76 | 0.326835 |
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Target: 5'- cGGUUCAGgugUCCGCGGUGCGAgaCGAu- -3' miRNA: 3'- -CCGAGUCa--AGGUGCCGCGCUg-GCUcu -5' |
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| 2989 | 5' | -57.4 | NC_001493.1 | + | 107448 | 1.11 | 0.001606 |
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Target: 5'- aGGCUCAGUUCCACGGCGCGACCGAGAu -3' miRNA: 3'- -CCGAGUCAAGGUGCCGCGCUGGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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