Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29894 | 3' | -54 | NC_006273.1 | + | 188991 | 0.66 | 0.982574 |
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Target: 5'- uGggGCUCGCGaucccuggGCCauGAUGGGGc--- -3' miRNA: 3'- uCuuUGAGCGCa-------CGG--CUGCCCCaaag -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 38227 | 0.66 | 0.987676 |
|
Target: 5'- cGGAAAC-CGCGgcGCCGgaggaaACGGGGa--- -3' miRNA: 3'- -UCUUUGaGCGCa-CGGC------UGCCCCaaag -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 92616 | 0.66 | 0.986124 |
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Target: 5'- cGGAAGC-CaGCGUGUaaucauaACGGGGUUUUg -3' miRNA: 3'- -UCUUUGaG-CGCACGgc-----UGCCCCAAAG- -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 147147 | 0.66 | 0.982574 |
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Target: 5'- cGAAAUgUCGCGUGUCGACu-GGUUUa -3' miRNA: 3'- uCUUUG-AGCGCACGGCUGccCCAAAg -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 195876 | 0.66 | 0.982574 |
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Target: 5'- ----uCUUGUGUGCCGAUGGGa---- -3' miRNA: 3'- ucuuuGAGCGCACGGCUGCCCcaaag -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 101698 | 0.66 | 0.980562 |
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Target: 5'- uGAGAC-CGC-UGCCGGgGGGcGggUCa -3' miRNA: 3'- uCUUUGaGCGcACGGCUgCCC-CaaAG- -5' |
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| 29894 | 3' | -54 | NC_006273.1 | + | 114515 | 0.66 | 0.982574 |
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Target: 5'- cGGcGACg-GCGUGCaCGGCGGGcGUUa- -3' miRNA: 3'- -UCuUUGagCGCACG-GCUGCCC-CAAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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