Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29897 | 3' | -65 | NC_006273.1 | + | 136494 | 0.66 | 0.642969 |
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Target: 5'- gAUCGCCguuugCCCGaAGCGGcuGUGgcuucucuggagacGGCCGUCa -3' miRNA: 3'- -UAGCGGa----GGGC-UCGCU--CGC--------------CCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 139111 | 0.66 | 0.597943 |
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Target: 5'- cGUCGCC-CUCGA-CG-GCGGuacugaaGCCGCCg -3' miRNA: 3'- -UAGCGGaGGGCUcGCuCGCC-------CGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 229519 | 0.66 | 0.589536 |
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Target: 5'- -aCGaCCcacgaCCCGAGcCGAaggaGGGCCGCCa -3' miRNA: 3'- uaGC-GGa----GGGCUC-GCUcg--CCCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 112537 | 0.66 | 0.589536 |
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Target: 5'- cGUCGCCgcCCCGucugacGCGGucgccGCGucagcGGCCGCCg -3' miRNA: 3'- -UAGCGGa-GGGCu-----CGCU-----CGC-----CCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 21249 | 0.66 | 0.589536 |
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Target: 5'- gAUCGCCUg--GAGCGAGUGGGgcaaCUGCUg -3' miRNA: 3'- -UAGCGGAgggCUCGCUCGCCC----GGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 82173 | 0.66 | 0.589536 |
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Target: 5'- --aGCCUgCUCGuuGGUGuaaGGCGGaGCCGCCg -3' miRNA: 3'- uagCGGA-GGGC--UCGC---UCGCC-CGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 120585 | 0.66 | 0.621373 |
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Target: 5'- -gCGUCUUgCCGguggcGGCGAcguuuggguugcgcaGCGGGCUGCCa -3' miRNA: 3'- uaGCGGAG-GGC-----UCGCU---------------CGCCCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 153783 | 0.66 | 0.617619 |
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Target: 5'- uUCgGCCUCCgccGCGGcCGcGGCCGCCg -3' miRNA: 3'- uAG-CGGAGGgcuCGCUcGC-CCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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