Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29897 | 3' | -65 | NC_006273.1 | + | 46308 | 0.75 | 0.200981 |
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Target: 5'- -cCGCCgCCCGGGCGcaagaAGC-GGCCGCCu -3' miRNA: 3'- uaGCGGaGGGCUCGC-----UCGcCCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 37668 | 0.67 | 0.561686 |
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Target: 5'- gGUCGUCaaCUGuGCuGAGCGGG-CGCCa -3' miRNA: 3'- -UAGCGGagGGCuCG-CUCGCCCgGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 23615 | 0.67 | 0.580219 |
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Target: 5'- cGUCGCCg-CCGAGCc--CGGGCCuGCUc -3' miRNA: 3'- -UAGCGGagGGCUCGcucGCCCGG-CGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 22255 | 0.67 | 0.534206 |
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Target: 5'- -gCGCCgg-CGAGCGAcGCGGcuCCGCCg -3' miRNA: 3'- uaGCGGaggGCUCGCU-CGCCc-GGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 21249 | 0.66 | 0.589536 |
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Target: 5'- gAUCGCCUg--GAGCGAGUGGGgcaaCUGCUg -3' miRNA: 3'- -UAGCGGAgggCUCGCUCGCCC----GGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 13570 | 0.7 | 0.401331 |
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Target: 5'- -aCGCCgggugcucacccgCCGGGCGGccCGGGCCGCCg -3' miRNA: 3'- uaGCGGag-----------GGCUCGCUc-GCCCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 1992 | 0.68 | 0.497467 |
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Target: 5'- cGUCGCg-CCagcGGCGAGCGgcacggagacggaGGCCGCCg -3' miRNA: 3'- -UAGCGgaGGgc-UCGCUCGC-------------CCGGCGG- -5' |
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| 29897 | 3' | -65 | NC_006273.1 | + | 1046 | 0.68 | 0.495703 |
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Target: 5'- -aCGCCgggccacggcguccaCCGGGUaGAaccccGCGGGCCGCCg -3' miRNA: 3'- uaGCGGag-------------GGCUCG-CU-----CGCCCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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