Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29897 | 5' | -58.5 | NC_006273.1 | + | 211301 | 0.66 | 0.862038 |
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Target: 5'- gGUGGCCCCggUgGCGCGUGacGAUAaccAGGUc -3' miRNA: 3'- -UACCGGGGg-AgCGUGCAC--CUAU---UCCA- -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 14676 | 0.67 | 0.839109 |
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Target: 5'- gGUGGCUaCCC-CGUACGUGGuuuuuauGGGUc -3' miRNA: 3'- -UACCGG-GGGaGCGUGCACCuau----UCCA- -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 143183 | 0.67 | 0.839109 |
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Target: 5'- uUGGCgCCCguaggCGCGCG-GGuuuuUGGGGUg -3' miRNA: 3'- uACCGgGGGa----GCGUGCaCCu---AUUCCA- -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 13960 | 0.67 | 0.839109 |
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Target: 5'- uUGGCCCUCcacaGCGCGuUGGAcGGGGc -3' miRNA: 3'- uACCGGGGGag--CGUGC-ACCUaUUCCa -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 105735 | 0.68 | 0.788684 |
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Target: 5'- -gGGCgUCCUCGCcCGUcgauagcucuuGGGUGAGGUu -3' miRNA: 3'- uaCCGgGGGAGCGuGCA-----------CCUAUUCCA- -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 147518 | 0.68 | 0.761586 |
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Target: 5'- -cGGCCCgCUCGCuCGggagGcGAUGGGGg -3' miRNA: 3'- uaCCGGGgGAGCGuGCa---C-CUAUUCCa -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 54996 | 0.69 | 0.72399 |
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Target: 5'- cAUGGCCUgCUCGUAgGUGGG--GGGc -3' miRNA: 3'- -UACCGGGgGAGCGUgCACCUauUCCa -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 83280 | 0.7 | 0.665536 |
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Target: 5'- cUGaCCCCCUCGCugGUGGAc----- -3' miRNA: 3'- uACcGGGGGAGCGugCACCUauucca -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 128672 | 0.74 | 0.427977 |
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Target: 5'- uUGGCCCCCgagcucguggUGUACGUGG-UGGGGUu -3' miRNA: 3'- uACCGGGGGa---------GCGUGCACCuAUUCCA- -5' |
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| 29897 | 5' | -58.5 | NC_006273.1 | + | 87904 | 1.05 | 0.004034 |
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Target: 5'- cAUGGCCCCCUCGCACGUGGAUAAGGUg -3' miRNA: 3'- -UACCGGGGGAGCGUGCACCUAUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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