Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2991 | 3' | -55.6 | NC_001493.1 | + | 112847 | 0.71 | 0.658053 |
Target: 5'- cGCCCguuccuccaggCCAUCGGggAUCUCGGG-CgCAGGc -3' miRNA: 3'- -UGGGa----------GGUAGCU--UAGAGCCCaG-GUCC- -5' |
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2991 | 3' | -55.6 | NC_001493.1 | + | 102177 | 0.72 | 0.62737 |
Target: 5'- cGCUCU-CAUCGGAUCUCGGa-CCGGGu -3' miRNA: 3'- -UGGGAgGUAGCUUAGAGCCcaGGUCC- -5' |
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2991 | 3' | -55.6 | NC_001493.1 | + | 106162 | 0.72 | 0.60692 |
Target: 5'- aGCCCUCCGUcaaCGAcUCaUCGGuGUaCCGGGg -3' miRNA: 3'- -UGGGAGGUA---GCUuAG-AGCC-CA-GGUCC- -5' |
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2991 | 3' | -55.6 | NC_001493.1 | + | 55271 | 0.75 | 0.459445 |
Target: 5'- cCCCUCUugaGAAUCUgCGGGaUCCAGGu -3' miRNA: 3'- uGGGAGGuagCUUAGA-GCCC-AGGUCC- -5' |
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2991 | 3' | -55.6 | NC_001493.1 | + | 108975 | 1.09 | 0.003024 |
Target: 5'- aACCCUCCAUCGAAUCUCGGGUCCAGGc -3' miRNA: 3'- -UGGGAGGUAGCUUAGAGCCCAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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