miRNA: 3'- caGUUAGUAGGacaUGCUUCUCCuua -5'
miRNA: 3'- -CAGU--UAGUAGGA-------CAuGCUUCUCCUUa -5'
miRNA: 3'- -CAGUUAgUAGGACAugCuuCuCC-UUa -5'
miRNA: 3'- cAGUUAGUa--GGACAUGCuuCUCCUua -5'
miRNA: 3'- -CAGUUAGUAGGACAUGCUUCUCCUUA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29925 | 5' | -47.1 | NC_006273.1 | + | 28366 | 0.67 | 0.999881 |
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Target: 5'- -aCAGUCGUCC---ACGAAGAGGu-- -3' miRNA: 3'- caGUUAGUAGGacaUGCUUCUCCuua -5' |
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| 29925 | 5' | -47.1 | NC_006273.1 | + | 89698 | 0.71 | 0.994362 |
|
Target: 5'- cGUCGucAUCGUCCUccccuucGUcCGAGGAGGAAg -3' miRNA: 3'- -CAGU--UAGUAGGA-------CAuGCUUCUCCUUa -5' |
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| 29925 | 5' | -47.1 | NC_006273.1 | + | 86102 | 0.71 | 0.993552 |
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Target: 5'- gGUCGAUgAUCCUGUugGggGcGGcAAa -3' miRNA: 3'- -CAGUUAgUAGGACAugCuuCuCC-UUa -5' |
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| 29925 | 5' | -47.1 | NC_006273.1 | + | 149186 | 0.77 | 0.899294 |
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Target: 5'- aUCAGUCGccaUCUGUACGggGAGGAc- -3' miRNA: 3'- cAGUUAGUa--GGACAUGCuuCUCCUua -5' |
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| 29925 | 5' | -47.1 | NC_006273.1 | + | 69283 | 1.06 | 0.045093 |
|
Target: 5'- uGUCAAUCAUCCUGUACGAAGAGGAAUc -3' miRNA: 3'- -CAGUUAGUAGGACAUGCUUCUCCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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