Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29928 | 5' | -51.7 | NC_006273.1 | + | 170333 | 0.71 | 0.940768 |
Target: 5'- aUACACGGCGGCGUuGUGacaUUCCGcggaGCUg -3' miRNA: 3'- aGUGUGCCGUUGUA-CAC---AAGGCa---CGA- -5' |
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29928 | 5' | -51.7 | NC_006273.1 | + | 37336 | 0.71 | 0.935964 |
Target: 5'- cUUugGCGGCAGCGgagGUGggCCGggGCc -3' miRNA: 3'- -AGugUGCCGUUGUa--CACaaGGCa-CGa -5' |
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29928 | 5' | -51.7 | NC_006273.1 | + | 119828 | 0.74 | 0.818771 |
Target: 5'- gUCGCACGGCc-CAgugGUGagCCGUGCg -3' miRNA: 3'- -AGUGUGCCGuuGUa--CACaaGGCACGa -5' |
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29928 | 5' | -51.7 | NC_006273.1 | + | 66256 | 1.09 | 0.010438 |
Target: 5'- gUCACACGGCAACAUGUGUUCCGUGCUg -3' miRNA: 3'- -AGUGUGCCGUUGUACACAAGGCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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