Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29929 | 5' | -59 | NC_006273.1 | + | 105264 | 0.66 | 0.848739 |
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Target: 5'- aAGGaUAGUCGUCCAGGugCaggcUGAGGu- -3' miRNA: 3'- cUCC-GUCGGUAGGUCCugG----ACUCCuu -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 84858 | 0.66 | 0.848739 |
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Target: 5'- -cGGCGGCCGUCaggguggAGGGCUUGgccacGGGAGc -3' miRNA: 3'- cuCCGUCGGUAGg------UCCUGGAC-----UCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 149905 | 0.66 | 0.819682 |
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Target: 5'- --cGCAGCCAauaccaCCAGGACCaagacgugguggacgUGGGGAAu -3' miRNA: 3'- cucCGUCGGUa-----GGUCCUGG---------------ACUCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 149569 | 0.66 | 0.816318 |
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Target: 5'- -uGGCGGCgG-CCGGGcCCgugGAGGAGg -3' miRNA: 3'- cuCCGUCGgUaGGUCCuGGa--CUCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 187308 | 0.66 | 0.807797 |
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Target: 5'- cGGGGCGGCUGcUCCAGGuCCgc-GGAGc -3' miRNA: 3'- -CUCCGUCGGU-AGGUCCuGGacuCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 190360 | 0.67 | 0.799125 |
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Target: 5'- cAGGCcGCCG-CCgugGGGGCCgcgGAGGGAg -3' miRNA: 3'- cUCCGuCGGUaGG---UCCUGGa--CUCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 161801 | 0.67 | 0.799125 |
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Target: 5'- -cGGCAGCgGcgggCCAGGACaucgGAGGGu -3' miRNA: 3'- cuCCGUCGgUa---GGUCCUGga--CUCCUu -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 110223 | 0.68 | 0.725272 |
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Target: 5'- aGAGuGCGGCCuggcgGUCUAGGGCCcGuGGGAc -3' miRNA: 3'- -CUC-CGUCGG-----UAGGUCCUGGaCuCCUU- -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 74401 | 0.69 | 0.686242 |
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Target: 5'- --cGCAGCUcgCUAGGACCcaacuUGAGGAu -3' miRNA: 3'- cucCGUCGGuaGGUCCUGG-----ACUCCUu -5' |
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| 29929 | 5' | -59 | NC_006273.1 | + | 64854 | 0.97 | 0.012421 |
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Target: 5'- cGAGGCAGCCAUCCAGG-CCUGAGGAAc -3' miRNA: 3'- -CUCCGUCGGUAGGUCCuGGACUCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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