Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29941 | 5' | -60 | NC_006273.1 | + | 139806 | 0.67 | 0.799557 |
Target: 5'- cCGUcagGUCuccgGGGuCCGCCGGGGUuucgacgucccugUGCUCg -3' miRNA: 3'- cGCAa--CAG----CCC-GGUGGUCCCG-------------ACGAG- -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 210499 | 0.66 | 0.808857 |
Target: 5'- aGCGUuuguuagcUGUCGGGUgAUCAGGGac-CUCc -3' miRNA: 3'- -CGCA--------ACAGCCCGgUGGUCCCgacGAG- -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 140641 | 0.66 | 0.808857 |
Target: 5'- aGCGggG-CGGGCCGggaAGGGgaGCUg -3' miRNA: 3'- -CGCaaCaGCCCGGUgg-UCCCgaCGAg -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 49949 | 0.66 | 0.81716 |
Target: 5'- aGCGU--UCGGGCCaggccacgugcaGCCGGGuGCcgcauuccUGCUCc -3' miRNA: 3'- -CGCAacAGCCCGG------------UGGUCC-CG--------ACGAG- -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 61774 | 0.66 | 0.841127 |
Target: 5'- uGCGUUGccgagcUCGGGCUACCAcuuuGGCuucgUGCgUCa -3' miRNA: 3'- -CGCAAC------AGCCCGGUGGUc---CCG----ACG-AG- -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 213102 | 0.66 | 0.848781 |
Target: 5'- aGCuGcUGUUcGGCCACCAGcgcguGGCUGCg- -3' miRNA: 3'- -CG-CaACAGcCCGGUGGUC-----CCGACGag -5' |
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29941 | 5' | -60 | NC_006273.1 | + | 155133 | 0.66 | 0.848781 |
Target: 5'- cGCGUUGuuUCGuGGCUaauGCCgAGGGCcGCg- -3' miRNA: 3'- -CGCAAC--AGC-CCGG---UGG-UCCCGaCGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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