Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29941 | 5' | -60 | NC_006273.1 | + | 83358 | 0.68 | 0.724746 |
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Target: 5'- gGCGccgUGgugggaggacccgCGGGCCACgucGGGCUGCUg -3' miRNA: 3'- -CGCa--ACa------------GCCCGGUGgu-CCCGACGAg -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 121608 | 0.69 | 0.690484 |
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Target: 5'- aUGUaGUC-GGCCA-CGGGGUUGCUCu -3' miRNA: 3'- cGCAaCAGcCCGGUgGUCCCGACGAG- -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 183699 | 0.69 | 0.68085 |
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Target: 5'- uCGUUGgcccacaucCaGGCCACCGGGGCUcagccuucucacGCUCc -3' miRNA: 3'- cGCAACa--------GcCCGGUGGUCCCGA------------CGAG- -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 198090 | 0.69 | 0.66148 |
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Target: 5'- cCGgcaUGUCcGGCCGUCgcaAGGGCUGCUCg -3' miRNA: 3'- cGCa--ACAGcCCGGUGG---UCCCGACGAG- -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 131269 | 0.72 | 0.480496 |
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Target: 5'- aGCGUcuUCGGGCaCACCgAGGGCcaggUGCUa -3' miRNA: 3'- -CGCAacAGCCCG-GUGG-UCCCG----ACGAg -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 43389 | 1.1 | 0.001735 |
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Target: 5'- gGCGUUGUCGGGCCACCAGGGCUGCUCc -3' miRNA: 3'- -CGCAACAGCCCGGUGGUCCCGACGAG- -5' |
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| 29941 | 5' | -60 | NC_006273.1 | + | 155133 | 0.66 | 0.848781 |
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Target: 5'- cGCGUUGuuUCGuGGCUaauGCCgAGGGCcGCg- -3' miRNA: 3'- -CGCAAC--AGC-CCGG---UGG-UCCCGaCGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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