Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29947 | 3' | -57 | NC_006273.1 | + | 112216 | 0.74 | 0.572626 |
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Target: 5'- --gCUGGAGGAcgcggcggugGCaAUGCGCGGCGGGGg -3' miRNA: 3'- uugGGCCUCCU----------UG-UGCGCGUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 113522 | 0.77 | 0.394675 |
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Target: 5'- uGCCCGGGGGGcGCGCGgGCGgcgaagagaagggcuACGGGGg -3' miRNA: 3'- uUGGGCCUCCU-UGUGCgCGU---------------UGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 140618 | 0.77 | 0.391417 |
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Target: 5'- aAGCCCGGAGGGGCccaaaaGC-CAGCGGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGug----CGcGUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 117972 | 0.77 | 0.407881 |
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Target: 5'- cACCUGGAGGAGC-CGUaGCGGCGGGa -3' miRNA: 3'- uUGGGCCUCCUUGuGCG-CGUUGUCCc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 234755 | 1.07 | 0.004868 |
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Target: 5'- gAACCCGGAGGAACACGCGCAACAGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGUGCGCGUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 39863 | 1.07 | 0.004868 |
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Target: 5'- gAACCCGGAGGAACACGCGCAACAGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGUGCGCGUUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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