Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29947 | 3' | -57 | NC_006273.1 | + | 215634 | 0.66 | 0.937562 |
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Target: 5'- cACCCaGAuaaGGAuguAgAUGCGCAACAGGa -3' miRNA: 3'- uUGGGcCU---CCU---UgUGCGCGUUGUCCc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 108625 | 0.66 | 0.937562 |
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Target: 5'- cGCCUcu-GGAcCACGCGCAGCgucAGGGc -3' miRNA: 3'- uUGGGccuCCUuGUGCGCGUUG---UCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 40928 | 0.66 | 0.942106 |
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Target: 5'- cGCUCGGGcGGACGCGUGCGGCGu-- -3' miRNA: 3'- uUGGGCCUcCUUGUGCGCGUUGUccc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 140838 | 0.66 | 0.942106 |
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Target: 5'- gAGCCCggGGAGGAGCGgGgUGgGGguGGGa -3' miRNA: 3'- -UUGGG--CCUCCUUGUgC-GCgUUguCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 149229 | 0.66 | 0.942106 |
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Target: 5'- uGCUCGG-GGAAaAgGCGUugGACGGGGa -3' miRNA: 3'- uUGGGCCuCCUUgUgCGCG--UUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 145309 | 0.66 | 0.942106 |
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Target: 5'- cACCUGGuGG-GC-UGCGCcGCGGGGc -3' miRNA: 3'- uUGGGCCuCCuUGuGCGCGuUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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