Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29947 | 3' | -57 | NC_006273.1 | + | 83469 | 0.7 | 0.793478 |
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Target: 5'- uGCCUGGAGGAGCAgauccaGuCGCAGguGGa -3' miRNA: 3'- uUGGGCCUCCUUGUg-----C-GCGUUguCCc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 40915 | 0.69 | 0.827329 |
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Target: 5'- cGGCCCGGuaacGGGAguUACGCugGgGACAGGGa -3' miRNA: 3'- -UUGGGCCu---CCUU--GUGCG--CgUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 198213 | 0.68 | 0.858536 |
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Target: 5'- uACCC-GAGGucuuuuGCACGCGCGACuuGGc -3' miRNA: 3'- uUGGGcCUCCu-----UGUGCGCGUUGucCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 156874 | 0.68 | 0.865875 |
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Target: 5'- cGCCCGGuguccGGGCG-GCGCcGCAGGGu -3' miRNA: 3'- uUGGGCCuc---CUUGUgCGCGuUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 203117 | 0.68 | 0.865875 |
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Target: 5'- -uCCCGcucgucgcguuGAGGAuaguCGCGCggugGCAGCGGGGg -3' miRNA: 3'- uuGGGC-----------CUCCUu---GUGCG----CGUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 39863 | 1.07 | 0.004868 |
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Target: 5'- gAACCCGGAGGAACACGCGCAACAGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGUGCGCGUUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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