Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29947 | 3' | -57 | NC_006273.1 | + | 93919 | 0.67 | 0.899534 |
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Target: 5'- gGACCCGGuGc-GCAUGCGCGGgcCAGGa -3' miRNA: 3'- -UUGGGCCuCcuUGUGCGCGUU--GUCCc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 132735 | 0.67 | 0.893221 |
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Target: 5'- gAGCgCCGG-GGAG-ACGaCGaCAGCAGGGa -3' miRNA: 3'- -UUG-GGCCuCCUUgUGC-GC-GUUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 141509 | 0.67 | 0.893221 |
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Target: 5'- gGACCUGGAGGuuGACgACGCcGUcuAACAGGu -3' miRNA: 3'- -UUGGGCCUCC--UUG-UGCG-CG--UUGUCCc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 113246 | 0.67 | 0.886695 |
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Target: 5'- gGACgauaaCGGAGGAGCugGUGCAAgAGc- -3' miRNA: 3'- -UUGg----GCCUCCUUGugCGCGUUgUCcc -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 996 | 0.67 | 0.886695 |
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Target: 5'- uACCUGGggacAGGGACGgggguUGCGCcgggGACGGGGg -3' miRNA: 3'- uUGGGCC----UCCUUGU-----GCGCG----UUGUCCC- -5' |
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| 29947 | 3' | -57 | NC_006273.1 | + | 234755 | 1.07 | 0.004868 |
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Target: 5'- gAACCCGGAGGAACACGCGCAACAGGGc -3' miRNA: 3'- -UUGGGCCUCCUUGUGCGCGUUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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