miRNA: 3'- aCCGGa-CGUUGCCGCa-------CCGa-------ACCCUU- -5'
miRNA: 3'- aCCGGaCGuUGCCGCaCCGaaCCCUU- -5'
miRNA: 3'- aCCGGA--CGUUGCC-GCACCGa-ACCCUU- -5'
miRNA: 3'- -ACCGGACGUUGCCGCACCGAACCCUU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29958 | 5' | -59.6 | NC_006273.1 | + | 73759 | 0.72 | 0.477613 |
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Target: 5'- aGGCCcgGCAGCGGCGccgccagcGGCgaccauggUGGGAGa -3' miRNA: 3'- aCCGGa-CGUUGCCGCa-------CCGa-------ACCCUU- -5' |
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| 29958 | 5' | -59.6 | NC_006273.1 | + | 211189 | 0.72 | 0.450571 |
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Target: 5'- cGGCCgGC-GCGGCG-GGUggGGGAAa -3' miRNA: 3'- aCCGGaCGuUGCCGCaCCGaaCCCUU- -5' |
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| 29958 | 5' | -59.6 | NC_006273.1 | + | 145617 | 0.73 | 0.433018 |
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Target: 5'- cGGUCUggGCGGCGGuCGUGGUggUGGGGGu -3' miRNA: 3'- aCCGGA--CGUUGCC-GCACCGa-ACCCUU- -5' |
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| 29958 | 5' | -59.6 | NC_006273.1 | + | 26642 | 1.05 | 0.002945 |
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Target: 5'- gUGGCCUGCAACGGCGUGGCUUGGGAAc -3' miRNA: 3'- -ACCGGACGUUGCCGCACCGAACCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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