Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29959 | 3' | -49.4 | NC_006273.1 | + | 91072 | 0.66 | 0.999597 |
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Target: 5'- uGCGCgACUgUCCGcgcgauUCCUuuuccagcaacUGGAAGAGc -3' miRNA: 3'- -UGCGaUGAaAGGCu-----AGGA-----------ACCUUCUC- -5' |
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| 29959 | 3' | -49.4 | NC_006273.1 | + | 67306 | 0.67 | 0.998866 |
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Target: 5'- gGCGCgccgGCgggaCUGGUCCUauaagagacUGGAGGAGc -3' miRNA: 3'- -UGCGa---UGaaa-GGCUAGGA---------ACCUUCUC- -5' |
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| 29959 | 3' | -49.4 | NC_006273.1 | + | 137247 | 0.67 | 0.998629 |
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Target: 5'- -aGCUGCUUUUCGGaCCgcGGAAcGAGa -3' miRNA: 3'- ugCGAUGAAAGGCUaGGaaCCUU-CUC- -5' |
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| 29959 | 3' | -49.4 | NC_006273.1 | + | 209119 | 0.68 | 0.99672 |
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Target: 5'- gACGCUG-UUUCCGAauaCCg-GGAGGAGa -3' miRNA: 3'- -UGCGAUgAAAGGCUa--GGaaCCUUCUC- -5' |
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| 29959 | 3' | -49.4 | NC_006273.1 | + | 56222 | 0.69 | 0.996388 |
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Target: 5'- gGCGCUGCgccgccuaaagugUCCGAUCCgcgcgGGAcgucgcugAGGGa -3' miRNA: 3'- -UGCGAUGaa-----------AGGCUAGGaa---CCU--------UCUC- -5' |
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| 29959 | 3' | -49.4 | NC_006273.1 | + | 26228 | 1.1 | 0.015514 |
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Target: 5'- gACGCUACUUUCCGAUCCUUGGAAGAGg -3' miRNA: 3'- -UGCGAUGAAAGGCUAGGAACCUUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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