miRNA: 3'- aCGCCAg-GGCugUACCUCUGUCAaag -5'
miRNA: 3'- -ACGCCaG-GGCUGUACC-UCUGUCAaag -5'
miRNA: 3'- -ACGCCAGGGCUGUACCUCUGUCAAAG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29960 | 3' | -53.6 | NC_006273.1 | + | 55086 | 0.71 | 0.879424 |
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Target: 5'- gGUGGUaaCUGugGUGGAGACGGUa-- -3' miRNA: 3'- aCGCCAg-GGCugUACCUCUGUCAaag -5' |
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| 29960 | 3' | -53.6 | NC_006273.1 | + | 71691 | 0.72 | 0.833557 |
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Target: 5'- aUGUGGcCgCCGACGUGGcAGGCGGUc-- -3' miRNA: 3'- -ACGCCaG-GGCUGUACC-UCUGUCAaag -5' |
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| 29960 | 3' | -53.6 | NC_006273.1 | + | 26134 | 1.09 | 0.007628 |
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Target: 5'- cUGCGGUCCCGACAUGGAGACAGUUUCc -3' miRNA: 3'- -ACGCCAGGGCUGUACCUCUGUCAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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