Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29972 | 3' | -55.1 | NC_006273.1 | + | 221796 | 0.66 | 0.96447 |
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Target: 5'- cUCCaGCACGGCggcccGGgccgCCcgGCGGGUGa -3' miRNA: 3'- aAGG-CGUGUUGa----CCaa--GGa-CGUCCAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 74594 | 0.66 | 0.961073 |
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Target: 5'- -gCCGCcCAACUGcGcgUCCcagGUAGGUGg -3' miRNA: 3'- aaGGCGuGUUGAC-Ca-AGGa--CGUCCAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 155252 | 0.67 | 0.940734 |
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Target: 5'- -aUCGCGCGGCUGGcuaaaaUUCCguUGCGGcGUGu -3' miRNA: 3'- aaGGCGUGUUGACC------AAGG--ACGUC-CAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 95739 | 0.67 | 0.935971 |
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Target: 5'- gUCCGCgACAACgUGGUUUU--CAGGUGg -3' miRNA: 3'- aAGGCG-UGUUG-ACCAAGGacGUCCAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 49946 | 0.67 | 0.925735 |
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Target: 5'- -aCCGCcgGCGACgcgaGaGUUCCUGCGcGGUGc -3' miRNA: 3'- aaGGCG--UGUUGa---C-CAAGGACGU-CCAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 46031 | 0.67 | 0.9197 |
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Target: 5'- -gCCGCugGaucuggaACUGGggCUcaUGCGGGUGg -3' miRNA: 3'- aaGGCGugU-------UGACCaaGG--ACGUCCAC- -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 43758 | 0.68 | 0.896006 |
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Target: 5'- gUUCCGCG----UGGUUCCUGCGcGGUu -3' miRNA: 3'- -AAGGCGUguugACCAAGGACGU-CCAc -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 100784 | 0.73 | 0.657117 |
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Target: 5'- -aCCGCGCGGCUGaugUCUUGCGGGa- -3' miRNA: 3'- aaGGCGUGUUGACca-AGGACGUCCac -5' |
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| 29972 | 3' | -55.1 | NC_006273.1 | + | 16102 | 1.05 | 0.007651 |
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Target: 5'- aUUCCGCACAACUGGUUCCUGCAGGUGc -3' miRNA: 3'- -AAGGCGUGUUGACCAAGGACGUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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