Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29982 | 5' | -50.3 | NC_006273.1 | + | 158479 | 0.66 | 0.99934 |
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Target: 5'- ----gUCCGCCAGGUGcACUuUGC-CUu -3' miRNA: 3'- agcaaAGGUGGUCCACuUGA-ACGaGA- -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 52856 | 0.66 | 0.99934 |
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Target: 5'- cCGagaaCGCCGGGUGAGCgcacaUGCUUUu -3' miRNA: 3'- aGCaaagGUGGUCCACUUGa----ACGAGA- -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 85532 | 0.66 | 0.99934 |
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Target: 5'- uUCGUUUUCACCGGGcUGGAagcGCa-- -3' miRNA: 3'- -AGCAAAGGUGGUCC-ACUUgaaCGaga -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 35087 | 0.66 | 0.99919 |
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Target: 5'- gCGUgugUUCC-CCAGGUGAggauggACUauaGCUCUc -3' miRNA: 3'- aGCA---AAGGuGGUCCACU------UGAa--CGAGA- -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 165774 | 0.67 | 0.997541 |
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Target: 5'- aCGUUauauauugcaUCCAgCAGGUGAGCcagGCUa- -3' miRNA: 3'- aGCAA----------AGGUgGUCCACUUGaa-CGAga -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 35943 | 0.67 | 0.997541 |
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Target: 5'- aCGUagCgCugCAGGUGAACcccGCUCg -3' miRNA: 3'- aGCAaaG-GugGUCCACUUGaa-CGAGa -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 18475 | 0.67 | 0.997541 |
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Target: 5'- cCGggUCgCACCgAGGUgGAGCcgUUGCUCa -3' miRNA: 3'- aGCaaAG-GUGG-UCCA-CUUG--AACGAGa -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 65088 | 0.68 | 0.992768 |
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Target: 5'- -gGUcUCCAUCAGGUGcuGAUggUGCUCa -3' miRNA: 3'- agCAaAGGUGGUCCAC--UUGa-ACGAGa -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 159339 | 0.69 | 0.990518 |
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Target: 5'- gCGgucgCgGCUAGGUGAGCUgUGUUCUa -3' miRNA: 3'- aGCaaa-GgUGGUCCACUUGA-ACGAGA- -5' |
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| 29982 | 5' | -50.3 | NC_006273.1 | + | 6299 | 1.08 | 0.016186 |
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Target: 5'- gUCGUUUCCACCAGGUGAACUUGCUCUc -3' miRNA: 3'- -AGCAAAGGUGGUCCACUUGAACGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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