Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29989 | 5' | -57.2 | NC_006273.1 | + | 227404 | 0.66 | 0.900333 |
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Target: 5'- cUGGGGAaaagaagGAgCUACCGGCUCAGGcGGc -3' miRNA: 3'- cAUCCCU-------CUaGAUGGUCGGGUCC-CUa -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 65572 | 0.66 | 0.894596 |
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Target: 5'- -aAGGGGcc-CUGCCGGCCCuGGGc- -3' miRNA: 3'- caUCCCUcuaGAUGGUCGGGuCCCua -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 208852 | 0.66 | 0.894596 |
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Target: 5'- aGUGGGGAGGUCguggaGGCaaCGGGGGUg -3' miRNA: 3'- -CAUCCCUCUAGaugg-UCGg-GUCCCUA- -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 140822 | 0.66 | 0.894596 |
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Target: 5'- -gAGGGA--UCccggGCCggAGCCCGGGGAg -3' miRNA: 3'- caUCCCUcuAGa---UGG--UCGGGUCCCUa -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 174946 | 0.68 | 0.819575 |
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Target: 5'- -gGGGGAGAugacagcgagcUCgaggagggGCCGcuGCCCGGGGGUg -3' miRNA: 3'- caUCCCUCU-----------AGa-------UGGU--CGGGUCCCUA- -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 140215 | 0.68 | 0.802388 |
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Target: 5'- -aGGGGAGugggggACCAcGCCCAGGGc- -3' miRNA: 3'- caUCCCUCuaga--UGGU-CGGGUCCCua -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 72914 | 0.7 | 0.724503 |
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Target: 5'- -gAGGGAGGcggccgccgcuccuUCUGCCGGUucaucaaCCGGGGAUc -3' miRNA: 3'- caUCCCUCU--------------AGAUGGUCG-------GGUCCCUA- -5' |
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| 29989 | 5' | -57.2 | NC_006273.1 | + | 2302 | 1.05 | 0.005682 |
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Target: 5'- gGUAGGGAGAUCUACCAGCCCAGGGAUc -3' miRNA: 3'- -CAUCCCUCUAGAUGGUCGGGUCCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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