Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30028 | 3' | -57.2 | NC_006273.1 | + | 189941 | 0.68 | 0.861903 |
Target: 5'- --cACAgCCGUCugcagcucgUCGGcCGGCGuGGGCGg -3' miRNA: 3'- auaUGU-GGCAG---------AGCCuGCCGC-CCUGC- -5' |
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30028 | 3' | -57.2 | NC_006273.1 | + | 169569 | 0.68 | 0.876069 |
Target: 5'- -cUGCGCCGcCgauugCGGACcGCaGGGGCGa -3' miRNA: 3'- auAUGUGGCaGa----GCCUGcCG-CCCUGC- -5' |
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30028 | 3' | -57.2 | NC_006273.1 | + | 204638 | 0.75 | 0.504022 |
Target: 5'- --gGCGCCGuUCUCGGGCaGCGaGGGCa -3' miRNA: 3'- auaUGUGGC-AGAGCCUGcCGC-CCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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