Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 30041 | 3' | -52.2 | NC_006273.1 | + | 196805 | 0.66 | 0.988769 |
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Target: 5'- ----uGCUGCgGCUGGcuGGCGCUgggcgcggugcUGCCc -3' miRNA: 3'- uucuuCGACGaCGACU--UCGUGA-----------ACGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 55386 | 0.66 | 0.990009 |
|
Target: 5'- cAGgcGCUGCUGC-GAcuccaccGGCACg-GCCc -3' miRNA: 3'- uUCuuCGACGACGaCU-------UCGUGaaCGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 61654 | 0.66 | 0.99014 |
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Target: 5'- --aGAGCaGCUGCg--GGCACggGCCa -3' miRNA: 3'- uucUUCGaCGACGacuUCGUGaaCGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 148650 | 0.66 | 0.991019 |
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Target: 5'- uGGAcGCgGCgGCUGAGGUGCUcucguggugcggccUGCCc -3' miRNA: 3'- uUCUuCGaCGaCGACUUCGUGA--------------ACGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 187308 | 0.66 | 0.991376 |
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Target: 5'- -cGggGCgGCUGCUccagguccgcGGAGCuaaUGCCg -3' miRNA: 3'- uuCuuCGaCGACGA----------CUUCGugaACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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