miRNA: 3'- -UUCUUCgACGACGACUUCGUga---ACGG- -5'
miRNA: 3'- -UUCUUcGACGACGAcuUCGUGAA-CGG- -5'
miRNA: 3'- -UUCuuCGACGAcGACUUCGuga-ACGG- -5'
miRNA: 3'- uUCUUCGaCGa-CGaCUUCGUGa-------ACGG- -5'
miRNA: 3'- uucuuCGACGaCGACU--UCGUGA-----------ACGG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30041 | 3' | -52.2 | NC_006273.1 | + | 8787 | 0.73 | 0.780147 |
|
Target: 5'- aAAGAGG-UGCUGCUGAAGUAagacgUGUCa -3' miRNA: 3'- -UUCUUCgACGACGACUUCGUga---ACGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 5706 | 0.68 | 0.971882 |
|
Target: 5'- cGGGGAcCUGUUGCUcuGGCGCUUcGCCc -3' miRNA: 3'- -UUCUUcGACGACGAcuUCGUGAA-CGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 4944 | 0.73 | 0.798896 |
|
Target: 5'- cGAGgcGCUGCU-CUGAAGCcaagUGCCg -3' miRNA: 3'- -UUCuuCGACGAcGACUUCGuga-ACGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 2100 | 0.68 | 0.968904 |
|
Target: 5'- cGGAGGC-GCcgGCgGGAGCGCgauuugcgUGCCa -3' miRNA: 3'- uUCUUCGaCGa-CGaCUUCGUGa-------ACGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 1913 | 0.66 | 0.988769 |
|
Target: 5'- ----uGCUGCgGCUGGcuGGCGCUgggcgcggugcUGCCc -3' miRNA: 3'- uucuuCGACGaCGACU--UCGUGA-----------ACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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