miRNA: 3'- uucUUCGACGa-----CGACuuCGUGaa-CGG- -5'
miRNA: 3'- -UUCUucGACGACGACuuCGUGaaCGG- -5'
miRNA: 3'- uUCUUCGa---------CGACGaCUUCGUGaa--CGG- -5'
miRNA: 3'- -UUCUUCGACGACGACuucGUGaa--CGG- -5'
miRNA: 3'- -UUCUUCGACGACGACuUCGUGaa---CGG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30041 | 3' | -52.2 | NC_006273.1 | + | 201950 | 0.68 | 0.958639 |
|
Target: 5'- --cAAGCUGCgccgggGCUGucGCGCgccGCCg -3' miRNA: 3'- uucUUCGACGa-----CGACuuCGUGaa-CGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 16308 | 0.68 | 0.965709 |
|
Target: 5'- aGAGAuuCUGCUGCUGu-GCGCgcGUCa -3' miRNA: 3'- -UUCUucGACGACGACuuCGUGaaCGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 95883 | 0.68 | 0.965709 |
|
Target: 5'- cAGGAGCgcuacgcggaGCUGCaGAAGCGCaagaGCCa -3' miRNA: 3'- uUCUUCGa---------CGACGaCUUCGUGaa--CGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 103892 | 0.68 | 0.965709 |
|
Target: 5'- cGAGGAGUUGCUGUUGca-CACcacgGCCg -3' miRNA: 3'- -UUCUUCGACGACGACuucGUGaa--CGG- -5' |
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| 30041 | 3' | -52.2 | NC_006273.1 | + | 190118 | 0.78 | 0.503436 |
|
Target: 5'- gAAGAGGUUGCUGUUGcGGCGCgagcaGCCg -3' miRNA: 3'- -UUCUUCGACGACGACuUCGUGaa---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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