Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3013 | 5' | -42.8 | NC_001493.1 | + | 43007 | 0.68 | 0.999988 |
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Target: 5'- ---gACACcuuGACuuaGUAUACAUGUGUg -3' miRNA: 3'- uauaUGUGu--CUGug-UAUAUGUACACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 126843 | 0.7 | 0.999885 |
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Target: 5'- ----cCGCGuACACAUGUAUAUGUGUc -3' miRNA: 3'- uauauGUGUcUGUGUAUAUGUACACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 11289 | 0.7 | 0.999885 |
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Target: 5'- ----cCGCGuACACAUGUAUAUGUGUc -3' miRNA: 3'- uauauGUGUcUGUGUAUAUGUACACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 15211 | 0.7 | 0.999847 |
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Target: 5'- --cUACugGGGCACAUc-GCGUGUGa -3' miRNA: 3'- uauAUGugUCUGUGUAuaUGUACACa -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 92879 | 0.7 | 0.999847 |
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Target: 5'- ---gACACAcGACcgugagcuccggGCGUAUGCGUGUGUc -3' miRNA: 3'- uauaUGUGU-CUG------------UGUAUAUGUACACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 130765 | 0.7 | 0.999847 |
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Target: 5'- --cUACugGGGCACAUc-GCGUGUGa -3' miRNA: 3'- uauAUGugUCUGUGUAuaUGUACACa -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 132350 | 0.72 | 0.998923 |
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Target: 5'- -gAUACACAGAUACAcggguccauagAUGCcgGUGUg -3' miRNA: 3'- uaUAUGUGUCUGUGUa----------UAUGuaCACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 16796 | 0.72 | 0.998923 |
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Target: 5'- -gAUACACAGAUACAcggguccauagAUGCcgGUGUg -3' miRNA: 3'- uaUAUGUGUCUGUGUa----------UAUGuaCACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 11455 | 1.07 | 0.05596 |
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Target: 5'- uAUAUACACAGACACAUAUACAUGUGUa -3' miRNA: 3'- -UAUAUGUGUCUGUGUAUAUGUACACA- -5' |
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| 3013 | 5' | -42.8 | NC_001493.1 | + | 127009 | 1.07 | 0.05596 |
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Target: 5'- uAUAUACACAGACACAUAUACAUGUGUa -3' miRNA: 3'- -UAUAUGUGUCUGUGUAUAUGUACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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