Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30140 | 5' | -57.8 | NC_006273.1 | + | 15670 | 0.66 | 0.915269 |
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Target: 5'- aCUGCCUgGUGACGaCGCGUUcGUCGa- -3' miRNA: 3'- -GAUGGAgUACUGC-GUGCGGcCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 131472 | 0.66 | 0.915269 |
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Target: 5'- -cGCuUUCGUGGCGUACGCCGuggcgcgcaaccGCCGc- -3' miRNA: 3'- gaUG-GAGUACUGCGUGCGGC------------CGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 55322 | 0.66 | 0.915269 |
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Target: 5'- cCUGCCgcguaUCggGAUGCACGCCGaagacggcGCCGc- -3' miRNA: 3'- -GAUGG-----AGuaCUGCGUGCGGC--------CGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 169807 | 0.66 | 0.915269 |
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Target: 5'- --cCCUCucGACGU-CGCCGGCUGUc -3' miRNA: 3'- gauGGAGuaCUGCGuGCGGCCGGCAa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 102825 | 0.66 | 0.915269 |
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Target: 5'- --uCgUCAUcGAaagGCGCGCCGGCCGc- -3' miRNA: 3'- gauGgAGUA-CUg--CGUGCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 188485 | 0.66 | 0.915269 |
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Target: 5'- -gGCCUCGggaGACGCGCGCa-GCCc-- -3' miRNA: 3'- gaUGGAGUa--CUGCGUGCGgcCGGcaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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