Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30140 | 5' | -57.8 | NC_006273.1 | + | 45342 | 0.73 | 0.530111 |
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Target: 5'- -cAgCUCAa-GCGCGCGCCGGCCGa- -3' miRNA: 3'- gaUgGAGUacUGCGUGCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 196673 | 0.74 | 0.51114 |
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Target: 5'- -aAUCUuggCGUGGCGCACGCagCGGCCGUa -3' miRNA: 3'- gaUGGA---GUACUGCGUGCG--GCCGGCAa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 186439 | 0.74 | 0.483234 |
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Target: 5'- gCUGCCUCGaaacgugaUGACGCACGaggaGGCCGa- -3' miRNA: 3'- -GAUGGAGU--------ACUGCGUGCgg--CCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 198169 | 0.75 | 0.447215 |
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Target: 5'- uCUGCCUgGgcACGCGCGUCGGCCGc- -3' miRNA: 3'- -GAUGGAgUacUGCGUGCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 101477 | 0.8 | 0.243467 |
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Target: 5'- uCUACCUCAUGGgcCGCgacaagGCGCUGGCCGUg -3' miRNA: 3'- -GAUGGAGUACU--GCG------UGCGGCCGGCAa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 135601 | 1.06 | 0.004912 |
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Target: 5'- gCUACCUCAUGACGCACGCCGGCCGUUa -3' miRNA: 3'- -GAUGGAGUACUGCGUGCGGCCGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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