Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30140 | 5' | -57.8 | NC_006273.1 | + | 198169 | 0.75 | 0.447215 |
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Target: 5'- uCUGCCUgGgcACGCGCGUCGGCCGc- -3' miRNA: 3'- -GAUGGAgUacUGCGUGCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 205605 | 0.69 | 0.763276 |
|
Target: 5'- -aACCUCAUGgaguuuGCGCGCGUCgGGCUGc- -3' miRNA: 3'- gaUGGAGUAC------UGCGUGCGG-CCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 206805 | 0.7 | 0.716188 |
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Target: 5'- -cGCCUCGcuuagaGCGCugGCCGGCUGc- -3' miRNA: 3'- gaUGGAGUac----UGCGugCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 212660 | 0.67 | 0.877569 |
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Target: 5'- gUACCUCGgcGACGCuccAUGCCGGCg--- -3' miRNA: 3'- gAUGGAGUa-CUGCG---UGCGGCCGgcaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 215698 | 0.69 | 0.790289 |
|
Target: 5'- -aGCgaCAUGGCGgcaACGCCGGCgGUUa -3' miRNA: 3'- gaUGgaGUACUGCg--UGCGGCCGgCAA- -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 222519 | 0.66 | 0.902961 |
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Target: 5'- -gGCgUCGUccacGGCGUagugaaaACGCCGGCCGg- -3' miRNA: 3'- gaUGgAGUA----CUGCG-------UGCGGCCGGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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