Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30140 | 5' | -57.8 | NC_006273.1 | + | 170614 | 0.66 | 0.890994 |
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Target: 5'- aCUGCCcCAcGGCGUAgGCCuucgcGGCCGUc -3' miRNA: 3'- -GAUGGaGUaCUGCGUgCGG-----CCGGCAa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 66618 | 0.67 | 0.870547 |
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Target: 5'- gCUACCUCAUGACGgGCaCUGuGCgCGa- -3' miRNA: 3'- -GAUGGAGUACUGCgUGcGGC-CG-GCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 56178 | 0.67 | 0.863325 |
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Target: 5'- --cCCUCuGUGACGCuguCGuCCGGCCa-- -3' miRNA: 3'- gauGGAG-UACUGCGu--GC-GGCCGGcaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 117439 | 0.67 | 0.863325 |
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Target: 5'- gCUACCguggCA-GGUGCGuCGCCGGCUGUg -3' miRNA: 3'- -GAUGGa---GUaCUGCGU-GCGGCCGGCAa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 75246 | 0.67 | 0.863325 |
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Target: 5'- gUACCUaugGACGaaGCGCUGGCCGc- -3' miRNA: 3'- gAUGGAguaCUGCg-UGCGGCCGGCaa -5' |
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| 30140 | 5' | -57.8 | NC_006273.1 | + | 135601 | 1.06 | 0.004912 |
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Target: 5'- gCUACCUCAUGACGCACGCCGGCCGUUa -3' miRNA: 3'- -GAUGGAGUACUGCGUGCGGCCGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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