Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30161 | 5' | -51 | NC_006273.1 | + | 188255 | 0.71 | 0.947593 |
Target: 5'- aUAGAUCGugGAgcaUGuCUCGCCaACUGu -3' miRNA: 3'- -GUCUAGUugCUag-AC-GAGCGGaUGAC- -5' |
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30161 | 5' | -51 | NC_006273.1 | + | 178429 | 0.72 | 0.917108 |
Target: 5'- cCAGccgCAACGGUCUGUUCGCCg---- -3' miRNA: 3'- -GUCua-GUUGCUAGACGAGCGGaugac -5' |
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30161 | 5' | -51 | NC_006273.1 | + | 153699 | 1.1 | 0.011736 |
Target: 5'- gCAGAUCAACGAUCUGCUCGCCUACUGg -3' miRNA: 3'- -GUCUAGUUGCUAGACGAGCGGAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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