miRNA: 3'- guaGGGGACGGa--CAGGu-UGGUGGUAc -5'
miRNA: 3'- guaGGGGACGGa--CAGGu-UGGUGGUAc -5'
miRNA: 3'- guaGGGGACGGacaGGUUGG--UGGUAC- -5'
miRNA: 3'- -GUAGGGGa--CGGA---CAGgUUGGUGGUAc -5'
miRNA: 3'- -GUAGGGGACGGACAGGUUGGUGGUAC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30164 | 5' | -56.3 | NC_006273.1 | + | 234228 | 0.69 | 0.825561 |
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Target: 5'- --gCCCCUGUCcccGUCCucACCACCGUc -3' miRNA: 3'- guaGGGGACGGa--CAGGu-UGGUGGUAc -5' |
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| 30164 | 5' | -56.3 | NC_006273.1 | + | 194290 | 0.69 | 0.825561 |
|
Target: 5'- --gCCCCUGUCcccGUCCucACCACCGUc -3' miRNA: 3'- guaGGGGACGGa--CAGGu-UGGUGGUAc -5' |
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| 30164 | 5' | -56.3 | NC_006273.1 | + | 64540 | 0.71 | 0.72592 |
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Target: 5'- --gUCCCUGCCg--CCGGCCucGCCAUGa -3' miRNA: 3'- guaGGGGACGGacaGGUUGG--UGGUAC- -5' |
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| 30164 | 5' | -56.3 | NC_006273.1 | + | 117259 | 0.75 | 0.518463 |
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Target: 5'- uCGUCCCCgucGCCUccaGUCgAACCGCCAUc -3' miRNA: 3'- -GUAGGGGa--CGGA---CAGgUUGGUGGUAc -5' |
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| 30164 | 5' | -56.3 | NC_006273.1 | + | 155143 | 1.09 | 0.004026 |
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Target: 5'- aCAUCCCCUGCCUGUCCAACCACCAUGa -3' miRNA: 3'- -GUAGGGGACGGACAGGUUGGUGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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