Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30166 | 3' | -56.4 | NC_006273.1 | + | 82181 | 0.7 | 0.780506 |
Target: 5'- -cGUugGUGUaagGCggaGCCGCCGUGGAUg- -3' miRNA: 3'- gaCGugCACA---CG---UGGUGGCACCUGau -5' |
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30166 | 3' | -56.4 | NC_006273.1 | + | 144313 | 0.71 | 0.733754 |
Target: 5'- -gGCgGCGuUGUGCGCgGCgGUGGGCUGu -3' miRNA: 3'- gaCG-UGC-ACACGUGgUGgCACCUGAU- -5' |
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30166 | 3' | -56.4 | NC_006273.1 | + | 201938 | 0.71 | 0.724116 |
Target: 5'- cCUGCACGUuuucaaGCugCGCCG-GGGCUGu -3' miRNA: 3'- -GACGUGCAca----CGugGUGGCaCCUGAU- -5' |
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30166 | 3' | -56.4 | NC_006273.1 | + | 142452 | 0.73 | 0.614934 |
Target: 5'- gUGCGCGUGUGCGCaCACgCGUG-ACg- -3' miRNA: 3'- gACGUGCACACGUG-GUG-GCACcUGau -5' |
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30166 | 3' | -56.4 | NC_006273.1 | + | 155666 | 0.97 | 0.022593 |
Target: 5'- uCUGCACGUGUGCACCACCGUGG-CUAc -3' miRNA: 3'- -GACGUGCACACGUGGUGGCACCuGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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