Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30167 | 5' | -60.1 | NC_006273.1 | + | 43017 | 0.7 | 0.625042 |
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Target: 5'- ---cGCCCucuGGGUgccGCGGGGggugugcGGGAGACGa -3' miRNA: 3'- uuuuUGGG---CCCA---CGCCCC-------CCCUCUGC- -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 140705 | 0.71 | 0.55576 |
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Target: 5'- uAGGAACCgGGGUcGCGacccagccccccguGGGGGGAGGgGa -3' miRNA: 3'- -UUUUUGGgCCCA-CGC--------------CCCCCCUCUgC- -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 212048 | 0.71 | 0.539682 |
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Target: 5'- ---cGCgaGGGUcGCGGGGGuGGGGGCGc -3' miRNA: 3'- uuuuUGggCCCA-CGCCCCC-CCUCUGC- -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 197930 | 0.72 | 0.493408 |
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Target: 5'- ----uCCCGGGUGCccgagacGGGGGGcAGAUGg -3' miRNA: 3'- uuuuuGGGCCCACGc------CCCCCC-UCUGC- -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 231436 | 0.78 | 0.242375 |
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Target: 5'- uGAAAGuCCCGuGUGgGGGGGGGGGGCa -3' miRNA: 3'- -UUUUU-GGGCcCACgCCCCCCCUCUGc -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 140836 | 0.83 | 0.107109 |
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Target: 5'- cGGAGCCCGGGgaggaGCGGGGuGGGGGugGg -3' miRNA: 3'- uUUUUGGGCCCa----CGCCCC-CCCUCugC- -5' |
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| 30167 | 5' | -60.1 | NC_006273.1 | + | 156371 | 1.07 | 0.002752 |
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Target: 5'- gAAAAACCCGGGUGCGGGGGGGAGACGg -3' miRNA: 3'- -UUUUUGGGCCCACGCCCCCCCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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