Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30168 | 3' | -53.6 | NC_006273.1 | + | 29874 | 0.66 | 0.990476 |
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Target: 5'- --aGGcacacaGCGGUCUUCUUCUgCUGAUc -3' miRNA: 3'- augCCag----CGCCAGAAGAAGA-GGCUAc -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 32450 | 0.66 | 0.989195 |
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Target: 5'- aGCGGUgUGUGGacgcucguccUCUgUCUUCUCCGGUu -3' miRNA: 3'- aUGCCA-GCGCC----------AGA-AGAAGAGGCUAc -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 161038 | 0.66 | 0.982691 |
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Target: 5'- gUACGGUuuauugCGaCGGUCuUUCUUUUCCGcGUGu -3' miRNA: 3'- -AUGCCA------GC-GCCAG-AAGAAGAGGC-UAC- -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 139867 | 0.67 | 0.970865 |
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Target: 5'- gACGGUCGUGGUCUccUCggCccgugCCGGg- -3' miRNA: 3'- aUGCCAGCGCCAGA--AGaaGa----GGCUac -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 51596 | 0.68 | 0.967935 |
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Target: 5'- gGCGGcagCGgGGUCUUCU--UCCGAc- -3' miRNA: 3'- aUGCCa--GCgCCAGAAGAagAGGCUac -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 87532 | 0.68 | 0.957904 |
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Target: 5'- cGCGG-CgGCGGUC-UCUgUCUCUGGUGc -3' miRNA: 3'- aUGCCaG-CGCCAGaAGA-AGAGGCUAC- -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 37250 | 0.69 | 0.94416 |
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Target: 5'- gGCGGUCuugGCGGUCgggUCUgucuuuuaugaugCUCCGAc- -3' miRNA: 3'- aUGCCAG---CGCCAGa--AGAa------------GAGGCUac -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 132568 | 0.7 | 0.921346 |
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Target: 5'- aGCGGUCGUuuuaaccgcaGGUCguccgUCUUCUCCa--- -3' miRNA: 3'- aUGCCAGCG----------CCAGa----AGAAGAGGcuac -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 147443 | 0.75 | 0.662054 |
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Target: 5'- gGgGGUgGCGGUCUUCUUCUCggCGAg- -3' miRNA: 3'- aUgCCAgCGCCAGAAGAAGAG--GCUac -5' |
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| 30168 | 3' | -53.6 | NC_006273.1 | + | 159173 | 1.08 | 0.009569 |
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Target: 5'- aUACGGUCGCGGUCUUCUUCUCCGAUGa -3' miRNA: 3'- -AUGCCAGCGCCAGAAGAAGAGGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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