Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30173 | 5' | -51.2 | NC_006273.1 | + | 57138 | 0.66 | 0.99783 |
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Target: 5'- aAUUCCAG-CUC--GCUGCCGUuggUCa -3' miRNA: 3'- gUGAGGUCaGAGauUGACGGCAca-AG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 196753 | 0.66 | 0.997427 |
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Target: 5'- gCACcCCGcGUCgcugCUGACgGCCGUGcgUCg -3' miRNA: 3'- -GUGaGGU-CAGa---GAUUGaCGGCACa-AG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 1861 | 0.66 | 0.997427 |
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Target: 5'- gCACcCCGcGUCgcugCUGACgGCCGUGcgUCg -3' miRNA: 3'- -GUGaGGU-CAGa---GAUUGaCGGCACa-AG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 99042 | 0.67 | 0.995143 |
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Target: 5'- aACUcCCAGUCacgaUAGCUGCCGUcGggCc -3' miRNA: 3'- gUGA-GGUCAGag--AUUGACGGCA-CaaG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 35733 | 0.67 | 0.993498 |
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Target: 5'- gAC-CCAGaUCUCgGGC-GCCGUGUUUg -3' miRNA: 3'- gUGaGGUC-AGAGaUUGaCGGCACAAG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 181377 | 0.67 | 0.992521 |
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Target: 5'- cCGCUCaCGGUCcgCUAugUccGCuCGUGUUCc -3' miRNA: 3'- -GUGAG-GUCAGa-GAUugA--CG-GCACAAG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 216473 | 0.71 | 0.951826 |
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Target: 5'- cCAgUCCAGagcgcUCUCUAGCUGCgagCGUGUg- -3' miRNA: 3'- -GUgAGGUC-----AGAGAUUGACG---GCACAag -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 200737 | 0.72 | 0.911172 |
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Target: 5'- cCGCUCCGGUCU--GGCUGCagcaGUGcUUCg -3' miRNA: 3'- -GUGAGGUCAGAgaUUGACGg---CAC-AAG- -5' |
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| 30173 | 5' | -51.2 | NC_006273.1 | + | 163187 | 1.09 | 0.011736 |
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Target: 5'- cCACUCCAGUCUCUAACUGCCGUGUUCc -3' miRNA: 3'- -GUGAGGUCAGAGAUUGACGGCACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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