Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30179 | 5' | -53.8 | NC_006273.1 | + | 127469 | 0.66 | 0.978612 |
Target: 5'- cUGCggu-GGUAGGGCGCCaucagccaGGUGUUc -3' miRNA: 3'- -ACGaacuUCAUCCCGUGGc-------CCACAAa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 82577 | 0.67 | 0.967864 |
Target: 5'- -cCUUGAGGUAGGGCGguagCGGGUc--- -3' miRNA: 3'- acGAACUUCAUCCCGUg---GCCCAcaaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 40797 | 0.68 | 0.940768 |
Target: 5'- cGCUcgGggGUu-GGCGgCGGGUGUUUc -3' miRNA: 3'- aCGAa-CuuCAucCCGUgGCCCACAAA- -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 859 | 0.68 | 0.940768 |
Target: 5'- cGCUcgGggGUu-GGCGgCGGGUGUUUc -3' miRNA: 3'- aCGAa-CuuCAucCCGUgGCCCACAAA- -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 150499 | 0.68 | 0.935964 |
Target: 5'- aGCUUGggGggugAGGGCAaauUgGGGUaGUUg -3' miRNA: 3'- aCGAACuuCa---UCCCGU---GgCCCA-CAAa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 119204 | 0.69 | 0.920104 |
Target: 5'- cGCUUGAuuuuGUccGGUuuACCGGGUGUg- -3' miRNA: 3'- aCGAACUu---CAucCCG--UGGCCCACAaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 38461 | 0.69 | 0.902076 |
Target: 5'- aGCaaGAGGUAGcGGCugCGGGUc--- -3' miRNA: 3'- aCGaaCUUCAUC-CCGugGCCCAcaaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 194935 | 0.72 | 0.782845 |
Target: 5'- cGCggcGggGUGGGuGUguGCCGGGUGUg- -3' miRNA: 3'- aCGaa-CuuCAUCC-CG--UGGCCCACAaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 42 | 0.72 | 0.782845 |
Target: 5'- cGCggcGggGUGGGuGUguGCCGGGUGUg- -3' miRNA: 3'- aCGaa-CuuCAUCC-CG--UGGCCCACAaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 234873 | 0.72 | 0.782845 |
Target: 5'- cGCggcGggGUGGGuGUguGCCGGGUGUg- -3' miRNA: 3'- aCGaa-CuuCAUCC-CG--UGGCCCACAaa -5' |
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30179 | 5' | -53.8 | NC_006273.1 | + | 168024 | 1.05 | 0.010732 |
Target: 5'- cUGCUUGAAGUAGGGCACCGGGUGUUUc -3' miRNA: 3'- -ACGAACUUCAUCCCGUGGCCCACAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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