Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30180 | 5' | -56.7 | NC_006273.1 | + | 8244 | 0.7 | 0.803595 |
Target: 5'- cGGGACg-GGGUGGGACGAgagCCGGUa- -3' miRNA: 3'- -UCCUGagCCCACUCUGCUg--GGCUAcu -5' |
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30180 | 5' | -56.7 | NC_006273.1 | + | 37440 | 0.71 | 0.778321 |
Target: 5'- uAGGAucuucgucgucaucCUCGGGUucggacgcgcacaacGGGAUGACCUGGUGGc -3' miRNA: 3'- -UCCU--------------GAGCCCA---------------CUCUGCUGGGCUACU- -5' |
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30180 | 5' | -56.7 | NC_006273.1 | + | 136089 | 0.74 | 0.605446 |
Target: 5'- -uGACUCGuGGUGAGAU-AUCCGAUGAu -3' miRNA: 3'- ucCUGAGC-CCACUCUGcUGGGCUACU- -5' |
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30180 | 5' | -56.7 | NC_006273.1 | + | 130015 | 0.76 | 0.472912 |
Target: 5'- cGGGCUCGGGUGAcGGCGACaucaCGGUc- -3' miRNA: 3'- uCCUGAGCCCACU-CUGCUGg---GCUAcu -5' |
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30180 | 5' | -56.7 | NC_006273.1 | + | 170197 | 1.09 | 0.004615 |
Target: 5'- cAGGACUCGGGUGAGACGACCCGAUGAa -3' miRNA: 3'- -UCCUGAGCCCACUCUGCUGGGCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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