miRNA: 3'- ucCUGAGCCCaCU-CUGCuGGGCUacu -5'
miRNA: 3'- uccugAGCCCACUCU-GCUGGGCUa-CU- -5'
miRNA: 3'- ucCUGa----GCCCACUCugC--UGGGC-UACU- -5'
miRNA: 3'- -UCCu-GAGCCCac-UCuGCUGGGCUaCU- -5'
miRNA: 3'- -UCcUGAGCCC-ACUCugCUGgGCuaCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30180 | 5' | -56.7 | NC_006273.1 | + | 177430 | 0.66 | 0.942063 |
|
Target: 5'- -uGACUCGGG-GAuGACG-CCCGAc-- -3' miRNA: 3'- ucCUGAGCCCaCU-CUGCuGGGCUacu -5' |
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| 30180 | 5' | -56.7 | NC_006273.1 | + | 185613 | 0.7 | 0.812042 |
|
Target: 5'- ----aUCGGGUGGGAgCGGCCCGGcgGAa -3' miRNA: 3'- uccugAGCCCACUCU-GCUGGGCUa-CU- -5' |
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| 30180 | 5' | -56.7 | NC_006273.1 | + | 189326 | 0.69 | 0.859336 |
|
Target: 5'- -cGGCgucggCGGGUGGGugGccACCCGcAUGAg -3' miRNA: 3'- ucCUGa----GCCCACUCugC--UGGGC-UACU- -5' |
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| 30180 | 5' | -56.7 | NC_006273.1 | + | 197776 | 0.68 | 0.905923 |
|
Target: 5'- uGGGucCUCGGGgacAGcCGACCCGAcGAu -3' miRNA: 3'- -UCCu-GAGCCCac-UCuGCUGGGCUaCU- -5' |
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| 30180 | 5' | -56.7 | NC_006273.1 | + | 232961 | 0.67 | 0.927888 |
|
Target: 5'- gAGuGCUCGGGaUGGGugGACgUGggGAc -3' miRNA: 3'- -UCcUGAGCCC-ACUCugCUGgGCuaCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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