Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30181 | 5' | -57.5 | NC_006273.1 | + | 14004 | 0.66 | 0.916029 |
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Target: 5'- cUGGggcgGGCgCGGGGUgGCGGA-GCg- -3' miRNA: 3'- uACCa---CCGaGCUCCAgUGCCUaCGag -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 4595 | 0.66 | 0.904293 |
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Target: 5'- gGUGGUGGCgcugGGGGUgGCGGuagugguacUGCUg -3' miRNA: 3'- -UACCACCGag--CUCCAgUGCCu--------ACGAg -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 163186 | 0.66 | 0.891669 |
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Target: 5'- cUGGUGGC-CGuGGUCuguCGGcaGUGCg- -3' miRNA: 3'- uACCACCGaGCuCCAGu--GCC--UACGag -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 150259 | 0.67 | 0.878179 |
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Target: 5'- -gGGUGGCUucCGAGGgccUGCGGuuuuuccgGCUCa -3' miRNA: 3'- uaCCACCGA--GCUCCa--GUGCCua------CGAG- -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 140708 | 0.67 | 0.871119 |
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Target: 5'- gGUGGUGGUggaGuGGUgGCGGcgGCg- -3' miRNA: 3'- -UACCACCGag-CuCCAgUGCCuaCGag -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 136807 | 0.68 | 0.840897 |
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Target: 5'- -cGGUGucucGCgucuccgCGAGGUCugGGAUGUg- -3' miRNA: 3'- uaCCAC----CGa------GCUCCAGugCCUACGag -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 51566 | 0.68 | 0.819682 |
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Target: 5'- gGUGGUGGCggcggaggacggaGGGGUgGCGGcgGCa- -3' miRNA: 3'- -UACCACCGag-----------CUCCAgUGCCuaCGag -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 144433 | 0.68 | 0.807797 |
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Target: 5'- -cGGacGGUUCG-GGUgGCGGcgGCUCg -3' miRNA: 3'- uaCCa-CCGAGCuCCAgUGCCuaCGAG- -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 173702 | 0.69 | 0.763081 |
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Target: 5'- --cGUGGC-CuuGGUCACGGGUGuCUCg -3' miRNA: 3'- uacCACCGaGcuCCAGUGCCUAC-GAG- -5' |
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| 30181 | 5' | -57.5 | NC_006273.1 | + | 171378 | 1.06 | 0.00433 |
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Target: 5'- cAUGGUGGCUCGAGGUCACGGAUGCUCc -3' miRNA: 3'- -UACCACCGAGCUCCAGUGCCUACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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