Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30188 | 5' | -54.1 | NC_006273.1 | + | 69262 | 0.66 | 0.983852 |
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Target: 5'- cGACCgccugcgUCGUCUaUGCUucGCGCGUCa--- -3' miRNA: 3'- aCUGG-------AGCAGA-ACGG--UGCGCAGcuuc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 102982 | 0.66 | 0.984032 |
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Target: 5'- -cGCCgCGUCgUGCCAacccgGCGUCGGAc -3' miRNA: 3'- acUGGaGCAGaACGGUg----CGCAGCUUc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 9166 | 0.66 | 0.985758 |
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Target: 5'- -cACCUCGUCggacaGCGUGUCGGAa -3' miRNA: 3'- acUGGAGCAGaacggUGCGCAGCUUc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 45328 | 0.66 | 0.985758 |
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Target: 5'- gGugUUCGUCcccCCGC-CGUCGGAGg -3' miRNA: 3'- aCugGAGCAGaacGGUGcGCAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 168691 | 0.66 | 0.985758 |
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Target: 5'- cGAcCCUCGUUUUGCUGCGUGg----- -3' miRNA: 3'- aCU-GGAGCAGAACGGUGCGCagcuuc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 115094 | 0.66 | 0.987336 |
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Target: 5'- gGAcCCUCGUCggaagcgGCCGCGaucagCGAGGc -3' miRNA: 3'- aCU-GGAGCAGaa-----CGGUGCgca--GCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 215025 | 0.66 | 0.987929 |
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Target: 5'- cGACCgUCGUCUgagcagugugggcgcUGCCGgGC-UCGGAa -3' miRNA: 3'- aCUGG-AGCAGA---------------ACGGUgCGcAGCUUc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 158649 | 0.65 | 0.988499 |
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Target: 5'- cGGCCUCGUUggugacgcggaugUUGCCggcgcauGCGgGUCGcAGa -3' miRNA: 3'- aCUGGAGCAG-------------AACGG-------UGCgCAGCuUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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