Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30188 | 5' | -54.1 | NC_006273.1 | + | 198271 | 0.69 | 0.940669 |
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Target: 5'- gGGCCUgCGgcguuaCUUGCgGCGCuUCGAGGg -3' miRNA: 3'- aCUGGA-GCa-----GAACGgUGCGcAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 197256 | 0.69 | 0.925935 |
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Target: 5'- cGGCCUCG-CcgGCCcgaGCGCGcCGggGa -3' miRNA: 3'- aCUGGAGCaGaaCGG---UGCGCaGCuuC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 35410 | 0.7 | 0.909139 |
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Target: 5'- uUGGCCUCGUCggccucgGCCACGUuccaCGAGc -3' miRNA: 3'- -ACUGGAGCAGaa-----CGGUGCGca--GCUUc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 143415 | 0.7 | 0.883592 |
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Target: 5'- -cGCCggcgCGgCcUGCCGCGCGUUGGAGa -3' miRNA: 3'- acUGGa---GCaGaACGGUGCGCAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 93850 | 0.71 | 0.846892 |
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Target: 5'- gUGGCCUCGag-UGCgCAUGCGcCGggGa -3' miRNA: 3'- -ACUGGAGCagaACG-GUGCGCaGCuuC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 200829 | 0.78 | 0.533868 |
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Target: 5'- gGACCUCGcugUCUggGCCGCGCuUCGAGGc -3' miRNA: 3'- aCUGGAGC---AGAa-CGGUGCGcAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 133396 | 0.78 | 0.495625 |
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Target: 5'- aGGCCUUGgaucCUccgccGCCGCGCGUCGAGGa -3' miRNA: 3'- aCUGGAGCa---GAa----CGGUGCGCAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 185865 | 1.08 | 0.008014 |
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Target: 5'- aUGACCUCGUCUUGCCACGCGUCGAAGc -3' miRNA: 3'- -ACUGGAGCAGAACGGUGCGCAGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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