Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30188 | 5' | -54.1 | NC_006273.1 | + | 162834 | 0.67 | 0.972949 |
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Target: 5'- gGGCCUCaUCgcaGCCuuGCGUCGGc- -3' miRNA: 3'- aCUGGAGcAGaa-CGGugCGCAGCUuc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 173462 | 0.68 | 0.964083 |
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Target: 5'- cGGuCCUCGUCUccGCCGC-CGUCGugcuGGu -3' miRNA: 3'- aCU-GGAGCAGAa-CGGUGcGCAGCu---UC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 39049 | 0.68 | 0.960722 |
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Target: 5'- cGGCCUUGUCgc-CCAC-CGUCGGGu -3' miRNA: 3'- aCUGGAGCAGaacGGUGcGCAGCUUc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 98630 | 0.68 | 0.953361 |
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Target: 5'- cGACCUCGUCgcgGUCGUGCGgaUCGGu- -3' miRNA: 3'- aCUGGAGCAGaa-CGGUGCGC--AGCUuc -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 198271 | 0.69 | 0.940669 |
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Target: 5'- gGGCCUgCGgcguuaCUUGCgGCGCuUCGAGGg -3' miRNA: 3'- aCUGGA-GCa-----GAACGgUGCGcAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 197256 | 0.69 | 0.925935 |
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Target: 5'- cGGCCUCG-CcgGCCcgaGCGCGcCGggGa -3' miRNA: 3'- aCUGGAGCaGaaCGG---UGCGCaGCuuC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 200829 | 0.78 | 0.533868 |
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Target: 5'- gGACCUCGcugUCUggGCCGCGCuUCGAGGc -3' miRNA: 3'- aCUGGAGC---AGAa-CGGUGCGcAGCUUC- -5' |
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| 30188 | 5' | -54.1 | NC_006273.1 | + | 158649 | 0.65 | 0.988499 |
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Target: 5'- cGGCCUCGUUggugacgcggaugUUGCCggcgcauGCGgGUCGcAGa -3' miRNA: 3'- aCUGGAGCAG-------------AACGG-------UGCgCAGCuUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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