Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30189 | 3' | -62.1 | NC_006273.1 | + | 151979 | 0.68 | 0.649825 |
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Target: 5'- cCGCUCGGGcGCCGCCgg---GCCGGg -3' miRNA: 3'- uGUGGGCCCuCGGCGGacuagCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 159734 | 0.68 | 0.630679 |
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Target: 5'- gUACUCGaGGAGCCaGCuCUGGUCGCa-- -3' miRNA: 3'- uGUGGGC-CCUCGG-CG-GACUAGCGguc -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 161641 | 0.7 | 0.499357 |
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Target: 5'- cGCugCCGGGccucGGCCGCCgc--CGCCAc -3' miRNA: 3'- -UGugGGCCC----UCGGCGGacuaGCGGUc -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 177679 | 0.67 | 0.659383 |
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Target: 5'- cGCGCCUcucGGAGCCGCC-GA-CGCUGGc -3' miRNA: 3'- -UGUGGGc--CCUCGGCGGaCUaGCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 185342 | 0.67 | 0.706745 |
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Target: 5'- uCGCCCGGGAuGcCCGCaaCUGAgCGuCCGGu -3' miRNA: 3'- uGUGGGCCCU-C-GGCG--GACUaGC-GGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 187027 | 1.08 | 0.001524 |
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Target: 5'- cACACCCGGGAGCCGCCUGAUCGCCAGg -3' miRNA: 3'- -UGUGGGCCCUCGGCGGACUAGCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 189443 | 0.79 | 0.155655 |
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Target: 5'- aGCACCCGGGAGCCGgCCUcgcGAcgaagucUCGUCGGa -3' miRNA: 3'- -UGUGGGCCCUCGGC-GGA---CU-------AGCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 200667 | 0.66 | 0.741863 |
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Target: 5'- cACGCCCGaGGAucuggcgGCCGCCggcGGUcagaaaaggaaaaCGCCGGc -3' miRNA: 3'- -UGUGGGC-CCU-------CGGCGGa--CUA-------------GCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 213120 | 0.67 | 0.697354 |
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Target: 5'- aGCGCaCCGacggcGGAGCCGCgUcGcUCGCCGGc -3' miRNA: 3'- -UGUG-GGC-----CCUCGGCGgA-CuAGCGGUC- -5' |
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| 30189 | 3' | -62.1 | NC_006273.1 | + | 214758 | 0.74 | 0.296453 |
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Target: 5'- aGCACCgUGGGuGCCGgCgguaUGAUCGCCAGu -3' miRNA: 3'- -UGUGG-GCCCuCGGCgG----ACUAGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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