Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30189 | 5' | -54.3 | NC_006273.1 | + | 44974 | 0.71 | 0.856888 |
Target: 5'- -cCCGUCGacguuguuuucUCCGAGCGGugaucGUUCCCGUCc -3' miRNA: 3'- caGGUAGC-----------AGGCUUGUU-----CGAGGGCAG- -5' |
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30189 | 5' | -54.3 | NC_006273.1 | + | 7753 | 0.72 | 0.816537 |
Target: 5'- -gCCGUCGaUCGAGCGGGCUCCgGg- -3' miRNA: 3'- caGGUAGCaGGCUUGUUCGAGGgCag -5' |
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30189 | 5' | -54.3 | NC_006273.1 | + | 133796 | 0.74 | 0.713061 |
Target: 5'- cUCCGUCGUuaucuuccCUGAGCGAGCgcgaacgccgcgCCCGUCg -3' miRNA: 3'- cAGGUAGCA--------GGCUUGUUCGa-----------GGGCAG- -5' |
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30189 | 5' | -54.3 | NC_006273.1 | + | 38752 | 0.76 | 0.605169 |
Target: 5'- -aCCGUCGUgCCGGcccaccgccgGCGcAGCUCCCGUCc -3' miRNA: 3'- caGGUAGCA-GGCU----------UGU-UCGAGGGCAG- -5' |
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30189 | 5' | -54.3 | NC_006273.1 | + | 233644 | 0.76 | 0.605169 |
Target: 5'- -aCCGUCGUgCCGGcccaccgccgGCGcAGCUCCCGUCc -3' miRNA: 3'- caGGUAGCA-GGCU----------UGU-UCGAGGGCAG- -5' |
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30189 | 5' | -54.3 | NC_006273.1 | + | 187065 | 1.11 | 0.005285 |
Target: 5'- cGUCCAUCGUCCGAACAAGCUCCCGUCa -3' miRNA: 3'- -CAGGUAGCAGGCUUGUUCGAGGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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