Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30189 | 5' | -54.3 | NC_006273.1 | + | 194309 | 0.69 | 0.913361 |
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Target: 5'- -aCCGUCGUCCGAuuACAAcaacgacgucauuucGCcUCCCaGUCa -3' miRNA: 3'- caGGUAGCAGGCU--UGUU---------------CG-AGGG-CAG- -5' |
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| 30189 | 5' | -54.3 | NC_006273.1 | + | 209180 | 0.66 | 0.982874 |
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Target: 5'- cGUCCGUCGUUuccgCGGACGcGGCUUCCc-- -3' miRNA: 3'- -CAGGUAGCAG----GCUUGU-UCGAGGGcag -5' |
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| 30189 | 5' | -54.3 | NC_006273.1 | + | 212121 | 0.66 | 0.980889 |
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Target: 5'- -gCCAagGUCgCGGAC-AGUUCCuCGUCg -3' miRNA: 3'- caGGUagCAG-GCUUGuUCGAGG-GCAG- -5' |
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| 30189 | 5' | -54.3 | NC_006273.1 | + | 215027 | 0.7 | 0.891695 |
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Target: 5'- -aCCGUCGUCUGAGCAGugugggcGCUgCCGg- -3' miRNA: 3'- caGGUAGCAGGCUUGUU-------CGAgGGCag -5' |
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| 30189 | 5' | -54.3 | NC_006273.1 | + | 233644 | 0.76 | 0.605169 |
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Target: 5'- -aCCGUCGUgCCGGcccaccgccgGCGcAGCUCCCGUCc -3' miRNA: 3'- caGGUAGCA-GGCU----------UGU-UCGAGGGCAG- -5' |
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| 30189 | 5' | -54.3 | NC_006273.1 | + | 234095 | 0.67 | 0.968314 |
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Target: 5'- aUCCGUacggcCGUCCGAGCGgcagcAGCgugCCCG-Cg -3' miRNA: 3'- cAGGUA-----GCAGGCUUGU-----UCGa--GGGCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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