Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30191 | 5' | -53.6 | NC_006273.1 | + | 74993 | 0.69 | 0.921618 |
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Target: 5'- -aUCAGCGGCGccGAUCG-CCAUGaauccgaguacccacGUGAg -3' miRNA: 3'- caAGUCGCCGU--CUAGCaGGUAC---------------CACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 50053 | 0.69 | 0.919393 |
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Target: 5'- -aUCAGCGugucccGUAGGUCGUCCAUGacgGAc -3' miRNA: 3'- caAGUCGC------CGUCUAGCAGGUACca-CU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 102477 | 0.7 | 0.907705 |
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Target: 5'- --gUAG-GGCAGAcguacagagCGUCCAUGGUGGu -3' miRNA: 3'- caaGUCgCCGUCUa--------GCAGGUACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 49148 | 0.7 | 0.901512 |
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Target: 5'- --cCAGaCGGUAGAcggCGUCCAUGGcGGg -3' miRNA: 3'- caaGUC-GCCGUCUa--GCAGGUACCaCU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 111360 | 0.7 | 0.895089 |
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Target: 5'- gGUUCAGCGGCGuGA---UCCAggUGGUGGu -3' miRNA: 3'- -CAAGUCGCCGU-CUagcAGGU--ACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 164967 | 0.71 | 0.881567 |
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Target: 5'- --gCAGCGGCGG---GUCCAgcUGGUGAc -3' miRNA: 3'- caaGUCGCCGUCuagCAGGU--ACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 90262 | 0.71 | 0.872306 |
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Target: 5'- -aUCGGCGGCGGcgaAUCGcCCgggcugccccgcagGUGGUGAu -3' miRNA: 3'- caAGUCGCCGUC---UAGCaGG--------------UACCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 33953 | 0.72 | 0.835925 |
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Target: 5'- ---aGGUGGCGGAUCG-CCGgcaGGUGAa -3' miRNA: 3'- caagUCGCCGUCUAGCaGGUa--CCACU- -5' |
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| 30191 | 5' | -53.6 | NC_006273.1 | + | 187741 | 1.09 | 0.007163 |
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Target: 5'- cGUUCAGCGGCAGAUCGUCCAUGGUGAc -3' miRNA: 3'- -CAAGUCGCCGUCUAGCAGGUACCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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